Experiment Protocol by Markdown Example ======================================= For today's experiment we will be analyzing the following blockquote: > ...the **twist** transcription factor binds broadly > across the genome and regulates important developmental > events. ### What we need To accomplish this we need three things from the following list: * an installed aligner * a working directory * a set of [fastq files](http://compgenomics/Data/ChIP-Seq/) The mutants are summarized in the following Table: Mutation | Dorsal | Result -------- | ------ | ------ gd7 | none | gd7_1.fastq.gz Tol10b | high | tol10b_1.fastq.gz The steps we will take are indicated in this ordrered list: 1. Create a working directory 2. write a simple alignment script * see the class examples 3. check the server *before* we run the script #### We also need to write some shell code our script might look something like: ```shell for f in FILES do tophat f done ``` We ran it on maple, which has ~~12 cores~~ 24 cores. ---