We have optimized the use of CRISPR/Cas9 in Nematostella vectensis
to generate INDELs in genes of interest for the lab. At present we are trying to use CRISPR/Cas9 to introduce sequences in the genome in a targeted manner. We have been successful in introducing sequences of interest, up to a length of 200 bp using single-strand DNA (ssDNA) as a donor. This allows us to tag genes of interest with small tags such as FLAG, HA, V5. Our next aim is to use bigger tags such as GFP, mScarlet among others. We are trying different donors: ssDNA, PCR products, PCR product with Biotin as a moiety in its 5’ and 3’ end, and other donors.
BS and MS in Biological Sciences, School of Sciences, University of Buenos Aires, Argentina.
Supervisor: Dr. Ruth Heinz.
Assistant supervisor: Dr. Andrea Puebla.
Master thesis title: “Cloning and characterization of genes of fructosyltranferases in Gramineae.”
PhD, School of Sciences, University of Buenos Aires, Argentina.
Supervisor: Dr. Ruth Heinz.
Assistant supervisor: Dr. Andrea Puebla
Thesis: “Fructan Metabolism in the Patagonian graminean species tolerant to abiotic stress: cloning and characterization of fructosyltransferases and fructan exohydrolases genes in BRomuspictuus”.
Postdoctoral Training, Yale School of Medicine, Yale University. Supervisor: Dr. Mustafa Khokha.
SELECTED PUBLICATIONSAnaxial Hox code controls tissue segmentation and body patterning in Nematostella vectensis.
He S, Del Viso F
, Chen CY, Ikmi A, Kroesen AE, Gibson MC.
. 2018 Sep28; 361(6409):1377-1380. doi: 10.1126/science.aar8384. PubMedRAPGEF5 Regulates Nuclear Translocation of β-Catenin.
Griffin JN, Del Viso F
, Duncan AR, Robson A, Hwang W, Kulkarni S, Liu KJ, Khokha MK.
2018 Jan 22;44(2):248-260.e4. doi:10.1016/j.devcel.2017.12.001. Epub 2017 Dec 28.PubMed
Congenital heart disease genetics uncovers context-dependent organization and function of nucleoporins at celia.Del Viso, F.
, Huang, F., Myers, J., Chalfant, M., Zhang, Y., Reza, N., Bewersdorf, J., Lusk, C.P., and Khokha, M.K.
. 2016. 38(5): 478-92.
PubMed Codon identity regulates mRNA stability and translation efficiency during the maternal-to-zygotic transition.
Bazzini, A., Del Viso, F.
, Moreno-Mateos, M.A., Johnstone, T.G., Vejnar, C.E., Qin, Y., Yao, J., Khokha, M.K., and Giraldez, A.J.
The EMBO Journal
. 2016. 35(19): 2087-2103.
Nucleoporin gene expression in Xenopus tropicalis embryonic development.
Reza, N., Khokha, M.K., and Del Viso, F.
The International Journal of Developmental Biology
. 2016. 60(4-6): 181-8.
The ribosome biogenesis factor Nol11 is required for optimal rDNA transcription and craniofacial development in Xenopus.
Griffin, J.N., Sondalle, S.B., Del Viso, F.
, Baserga, S.J., and Khokha, M.K.
. 2015. 11(3): e1005018.
PubMed Exon capture and bulk segregant analysis: rapid discovery of causative mutations using high-throughput sequencing.Del Viso, F.
, Bhattacharya, D., Kong, Y., Gilchrist, M.J., and Khokha, M.K.
. 2012. 13(1): 649.
PubMed Generating diploid embryos from Xenopus tropicalis.Del Viso, F.
, and Khokha, M.K.
Methods in Molecular Biology
. 2012. 917: 33-41.