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FLORENCIA DEL VISO
Research Specialist
RESEARCH SUMMARY
We have optimized the use of CRISPR/Cas9 in Nematostella vectensis to generate INDELs in genes of interest for the lab. At present we are trying to use CRISPR/Cas9 to introduce sequences in the genome in a targeted manner. We have been successful in introducing sequences of interest, up to a length of 200 bp using single-strand DNA (ssDNA) as a donor. This allows us to tag genes of interest with small tags such as FLAG, HA, V5. Our next aim is to use bigger tags such as GFP, mScarlet among others. We are trying different donors: ssDNA, PCR products, PCR product with Biotin as a moiety in its 5’ and 3’ end, and other donors.


EDUCATION
1997-2003  BS and MS in Biological Sciences, School of Sciences, University of Buenos Aires, Argentina.
Supervisor: Dr. Ruth Heinz.
Assistant supervisor: Dr. Andrea Puebla.
Master thesis title: “Cloning and characterization of genes of fructosyltranferases in Gramineae.”  

2004-2009    PhD, School of Sciences, University of Buenos Aires, Argentina.
Supervisor: Dr. Ruth Heinz.
Assistant supervisor: Dr. Andrea Puebla
Thesis: “Fructan Metabolism in the Patagonian graminean species tolerant to abiotic stress: cloning and characterization of fructosyltransferases and fructan exohydrolases  genes in BRomuspictuus”. 

2010-2015   Postdoctoral Training, Yale School of Medicine, Yale University. Supervisor: Dr. Mustafa Khokha.

SELECTED PUBLICATIONS
Anaxial Hox code controls tissue segmentation and body patterning in Nematostella vectensis.
He S, Del Viso F, Chen CY, Ikmi A, Kroesen AE, Gibson MC.
Science
. 2018 Sep28; 361(6409):1377-1380. doi: 10.1126/science.aar8384. 
PubMed

RAPGEF5 Regulates Nuclear Translocation of β-Catenin.
Griffin JN, Del Viso F, Duncan AR, Robson A, Hwang W, Kulkarni S, Liu KJ, Khokha MK.
Dev Cell. 2018 Jan 22;44(2):248-260.e4. doi:10.1016/j.devcel.2017.12.001. Epub 2017 Dec 28.
PubMed

Congenital heart disease genetics uncovers context-dependent organization and function of nucleoporins at celia.
Del Viso, F., Huang, F., Myers, J., Chalfant, M., Zhang, Y., Reza, N., Bewersdorf, J., Lusk, C.P., and Khokha, M.K.
Developmental Cell. 2016. 38(5): 478-92.
PubMed

Codon identity regulates mRNA stability and translation efficiency during the maternal-to-zygotic transition.
Bazzini, A., Del Viso, F., Moreno-Mateos, M.A., Johnstone, T.G., Vejnar, C.E., Qin, Y., Yao, J., Khokha, M.K., and Giraldez, A.J.
The EMBO Journal. 2016. 35(19): 2087-2103.
PubMed

Nucleoporin gene expression in Xenopus tropicalis embryonic development.
Reza, N., Khokha, M.K., and Del Viso, F.
The International Journal of Developmental Biology. 2016. 60(4-6): 181-8.
PubMed

The ribosome biogenesis factor Nol11 is required for optimal rDNA transcription and craniofacial development in Xenopus.
Griffin, J.N., Sondalle, S.B., Del Viso, F., Baserga, S.J., and Khokha, M.K.
PLOS Genetics. 2015. 11(3): e1005018.
PubMed

Exon capture and bulk segregant analysis: rapid discovery of causative mutations using high-throughput sequencing.
Del Viso, F., Bhattacharya, D., Kong, Y., Gilchrist, M.J., and Khokha, M.K.
BMC Genomics. 2012. 13(1): 649.
PubMed

Generating diploid embryos from Xenopus tropicalis.
Del Viso, F., and Khokha, M.K.
Methods in Molecular Biology. 2012. 917: 33-41.
PubMed