Dual Color N&B Calculators

Introduction

Brightnesses of Independent Species are Known (G(0) Version)

Input Values
Bleedthrough Fraction	Green Monomer Brightness	Red Monomer Brightness
Green Channel G(0)	Red Channel G(0)	Cross Corr G(0)
Green Intensity	Red Intensity	Gamma Factor
Output Values
N Green Monomer	N Red Monomer	N Complex
Green Stoichiometry	Red Stoichiometry

Brightnesses of Independent Species are Known (Brightness Version)

Input Values
Bleedthrough Fraction	Green Monomer Brightness	Red Monomer Brightness
Green Channel Brightness	Red Channel Brightness	Dual Color Brightness
Green Intensity	Red Intensity
Output Values
N Green Monomer	N Red Monomer	N Complex
Green Stoichiometry	Red Stoichiometry

Stoichiometry of Complex is Known (G(0) Version)

Input Values
Bleedthrough Fraction	Green Stoichiometry	Red Stoichiometry
Green Channel G(0)	Red Channel G(0)	Cross Corr G(0)
Green Intensity	Red Intensity	Gamma Factor
Output Values
N Green Monomer	N Red Monomer	N Complex
Green Monomer Brightness	Red Monomer Brightness

Stoichiometry of Complex is Known (Brightness Version)

Input Values
Bleedthrough Fraction	Green Stoichiometry	Red Stoichiometry
Green Channel Brightness	Red Channel Brightness	Dual Color Brightness
Green Intensity	Red Intensity
Output Values
N Green Monomer	N Red Monomer	N Complex
Green Monomer Brightness	Red Monomer Brightness

About the Calculators

The calculators are built using html forms and javascript. If you look at the source code for this page, you should be able to find java script functions like jcalc...(). The functions used for these calculations are listed below.






Note that n_g (the stoichiometry of the green species) is dependent on n_r. In the same way, ε_gm (brightness of the green monomer) is dependent on ε_rm. β is the green to red bleedthrough fraction mentioned above. For calculations involving G(0), the amplitudes are converted first to brightnesses (see the Brightness Analysis page) and then the calculation proceeds with the equations above.