CRISPR-Cas13d induces efficient mRNA knockdown in animal embryos

We demonstrated that CRISPR-RfxCas13d is an effective and precise system to deplete specific mRNA transcripts in zebrafish embryos. Both zygotically-expressed and maternally-provided transcripts can be efficiently targeted in zebrafish embryos, resulting in a 75% average decrease in transcript levels and the generate developmental phenotypes. Moreover, CRISPR-RfxCas13d can be used in medaka, killifish and mouse embryos.
We collaborate with our friend More (Miguel Moreno-Mateos, Centro Andaluz de Biologia del Desarrollo) to discover, improve and use CRISPR-Cas13d in embryos.