Visualizing recombination and the synaptonemal complex in D. melanogaster females

Mapping of synaptonemal complex (SC) along chromosome arms in a single nucleus within the germarium of a female ovary. Each chromosome arm is marked with dots of the same color. The SC is labeled using an antibody to the central region protein C(3)G (green), and Vilya3XHA foci are identified with an antibody to HA (red). These foci are part of recombination nodules that are the epicenter for recombination during Drosophila female meiosis. For more information, see our recent publication in ELife.

Lake, C. M., Nielsen, R. J., Guo, F., Unruh, J. R., Slaughter, B. D., & Hawley, R. S. (2015). Vilya, a component of the recombination nodule, is required for meiotic double-strand break formation in Drosophila. ELife, 4(OCTOBER2015), e08287.